バイオ研究とバイオ研究者の情報チャンネル
                   biolohas.jp

 

20091221
siRNA

26163



Transduction of RNA-directed DNA methylation signals to repressive histone marks in Arabidopsis thaliana.
Numa H, Kim JM, Matsui A, Kurihara Y, Morosawa T, Ishida J, Mochizuki Y, Kimura H, Shinozaki K, Toyoda T, Seki M, Yoshikawa M, Habu Y.
EMBO J. . [Epub ahead of print]PMID: 20010696
転写抑制とDNAのメチル化


RNAi-mediated viral immunity requires amplification of virus-derived siRNAs in Arabidopsis thaliana.
Wang XB, Wu Q, Ito T, Cillo F, Li WX, Chen X, Yu JL, Ding SW.
Proc Natl Acad Sci U S A. 2009 Dec 4. [Epub ahead of print]PMID: 19966292
RNAiが媒介する、ウィルス免疫


Analysis of acyclic nucleoside modifications in siRNAs finds sensitivity at position 1 that is restored by 5'-terminal phosphorylation both in vitro and in vivo.
Kenski DM, Cooper AJ, Li JJ, Willingham AT, Haringsma HJ, Young TA, Kuklin NA, Jones JJ, Cancilla MT, McMasters DR, Mathur M, Sachs AB, Flanagan WM.
Nucleic Acids Res. 2009 Nov 16.
PMID: 19917641
siRNAに修飾塩基を使うときは、5'をリン酸化するといいかもしれない


Inhibition of Hsp27 and Hsp40 potentiates 5-fluorouracil and carboplatin mediated cell killing in hepatoma cells.
Sharma A, Upadhyay AK, Bhat MK.
Cancer Biol Ther. 2009 Nov 3;8(22).
PMID: 19901540
抗がん剤の治療の際のsiRNA利用

Chapter 17 - Engineering cationic liposome siRNA complexes for in vitro and in vivo delivery.
Podesta JE, Kostarelos K.
Methods Enzymol. 2009;464:343-54.
PMID: 19903563

Chapter 14 Targeted Lipoplexes for siRNA Delivery.
Cardoso A, Trabulo S, Moreira JN, Duzgune? N, de Lima MC.
Methods Enzymol. 2009;465:267-87.
PMID: 19913172

siRNAの導入方法


Loss of the mammalian APC/C activator FZR1 shortens G1 and lengthens S phase but has little effect on exit from mitosis.
Sigl R, Wandke C, Rauch V, Kirk J, Hunt T, Geley S.
J Cell Sci. 2009 Oct 27. [Epub ahead of print]
PMID: 19861496

siRNAで細胞周期を解析

Novel nuclear nesprin-2 variants tether active extracellular signal-regulated MAPKs 1 and 2 at promyelocytic leukaemia protein nuclear bodies and act to regulate smooth muscle cell proliferation.
Warren DT, Tajsic T, Mellad JA, Searles R, Zhang Q, Shanahan CM.
J Biol Chem. 2009 Oct 27.
PMID: 19861416
プルダウンアッセイと、RNAiとの組み合わせで


Mediator of DNA damage checkpoint 1 (MDC1) regulates mitotic progression.
Townsend K, Dyson H, Blackford AN, Miller ES, Chapman JR, Sedgwick GG, Barone G, Turnell AS, Stewart GS.
J Biol Chem. 2009 Oct 13.
PMID: 19826003
siRNAで細胞分裂を止める


siRNA delivery not Toll-free.
Gantier MP, Williams BR.
Nat Biotechnol. 2009 Oct;27(10):911-2. No abstract available.
PMID: 19816445
コメント


Solid polymeric microparticles enhance the delivery of siRNA to macrophages in vivo.
Lee S, Yang SC, Kao CY, Pierce RH, Murthy N.
Nucleic Acids Res. 2009 Sep 25.
PMID: 19783825
siRNAの輸送方法

Adenosine-to-inosine genetic recoding is required in the adult-stage nervous system for coordinated behavior in Drosophila.
Jepson JE, Reenan RA.
J Biol Chem. 2009 Sep 15.
PMID: 19759011
ショウジョウバエの神経系でRNAエディティングが起こるが、行動には影響しない


RNAi in Budding Yeast.
Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP.
Science. 2009 Sep 10.
PMID: 19745116
出芽酵母のRNAi


An RNA-dependent RNA polymerase formed by TERT and the RMRP RNA.
Maida Y, Yasukawa M, Furuuchi M, Lassmann T, Possemato R, Okamoto N, Kasim V, Hayashizaki Y, Hahn WC, Masutomi K.
Nature. 2009 Aug 23. [Epub ahead of print]
PMID: 19701182
RdRPがテロメアや遺伝病の因子と相互作用する


Suppression of induced pluripotent stem cell generation by the p53-p21 pathway.
Hong H, Takahashi K, Ichisaka T, Aoi T, Kanagawa O, Nakagawa M, Okita K, Yamanaka S.
Nature. 2009 Aug 9.
PMID: 19668191
もう皆さんご存じだと思いますが、この号に複数の論文があります。
iPS細胞を高頻度で作り出す技術。


Identification of genes conferring resistance to 5-fluorouracil.
Yoo BK, Gredler R, Vozhilla N, Su ZZ, Chen D, Forcier T, Shah K, Saxena U, Hansen U, Fisher PB, Sarkar D.
Proc Natl Acad Sci U S A. 2009 Jul 21.
PMID: 19622726
5-FU耐性遺伝子をsiRNAで確認

Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton.
Dawe HR, Adams M, Wheway G, Szymanska K, Logan CV, Noegel AA, Gull K, Johnson CA.
J Cell Sci. 2009 Aug 1;122(Pt 15):2716-26. Epub 2009 Jul 13.
PMID: 19596800
細胞内の染色体やオルガネラが不安定化する病気


Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression.
Khalil AM, Guttman M, Huarte M, Garber M, Raj A, Rivea Morales D, Thomas K, Presser A, Bernstein BE, van Oudenaarden A, Regev A, Lander ES, Rinn JL.
Proc Natl Acad Sci U S A. 2009 Jul 1.
PMID: 19571010
多数のlincRNAがクロマチンを変化させて、遺伝子の発現制御をしている

Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug.
MacDiarmid JA, Amaro-Mugridge NB, Madrid-Weiss J, Sedliarou I, Wetzel S, Kochar K, Brahmbhatt VN, Phillips L, Pattison ST, Petti C, Stillman B, Graham RM, Brahmbhatt H.
Nat Biotechnol. 2009 Jul;27(7):643-51. Epub 2009 Jun 28.
PMID: 19561595
抗がん剤の治療にsiRNAや細胞表面たんぱく質に対する抗体を併用する


Synthetic heterochromatin bypasses RNAi and centromeric repeats to establish functional centromeres.
Kagansky A, Folco HD, Almeida R, Pidoux AL, Boukaba A, Simmer F, Urano T, Hamilton GL, Allshire RC.
Science. 2009 Jun 26;324(5935):1716-9.
PMID: 19556509
セントロメアの領域は、RNAiが起こりにくい


Genome-wide demethylation of Arabidopsis endosperm.
Hsieh TF, Ibarra CA, Silva P, Zemach A, Eshed-Williams L, Fischer RL, Zilberman D.
Science. 2009 Jun 12;324(5933):1451-4.
PMID: 19520962
アラビドの内胚乳における脱メチル化

siRNA Delivery and Targeting.
Cheng K, Mahato RI.
Mol Pharm. 2009 Jun-Jul;6(3):649-50. No abstract available.
PMID: 19485323
レビュー


Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs.
Khan AA, Betel D, Miller ML, Sander C, Leslie CS, Marks DS.
Nat Biotechnol. 2009 May 24.
PMID: 19465925
siRNAの導入で、内在性のmiRNAを阻害して、遺伝子の発現が上昇することも起こる

Promoter-specific transcriptional interference and c-myc gene silencing by siRNAs in human cells.
Napoli S, Pastori C, Magistri M, Carbone GM, Catapano CV.
EMBO J. 2009 May 21.
PMID: 19461583
siRNAによる転写そのものの阻害

Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein.
Eguchi A, Meade BR, Chang YC, Fredrickson CT, Willert K, Puri N, Dowdy SF.
Nat Biotechnol. 2009 May 17.
PMID: 19448630
融合タンパク質による、siRNA導入の効率化


An effector of RNA-directed DNA methylation in arabidopsis is an ARGONAUTE 4- and RNA-binding protein.
He XJ, Hsu YF, Zhu S, Wierzbicki AT, Pontes O, Pikaard CS, Liu HL, Wang CS, Jin H, Zhu JK.
Cell. 2009 May 1;137(3):498-508.
PMID: 19410546
siRNAとDNAメチル化

Intravaginal gene silencing using biodegradable polymer nanoparticles densely loaded with small-interfering RNA.
Woodrow KA, Cu Y, Booth CJ, Saucier-Sawyer JK, Wood MJ, Mark Saltzman W.
Nat Mater. 2009 May 3.
PMID: 19404239
siRNAのデリバリー

Gold, Poly(beta-amino ester) Nanoparticles for Small Interfering RNA Delivery.
Lee JS, Green JJ, Love KT, Sunshine J, Langer R, Anderson DG.
Nano Lett. 2009 May 7.
PMID: 19422265
ナノパーティクルでsiRNAをデリバリー


The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line.
The FANTOM Consortium, Suzuki H et al.
Nat Genet. 2009 Apr 19. [Epub ahead of print]
PMID: 19377474
52種類の転写因子をノックダウン

Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J.
Proc Natl Acad Sci U S A. 2009 Apr 9.
PMID: 19359485
短いsmall RNAが血管新生を抑える


Small RNA deep sequencing reveals role for Arabidopsis thaliana RNA-dependent RNA polymerases in viral siRNA biogenesis.
Qi X, Bao FS, Xie Z.
PLoS ONE. 2009;4(3):e4971. Epub 2009 Mar 24.
PMID: 19308254
RNAウィルス防御のためのRDRの役割


Autophagy enhances the efficacy of BCG vaccine by increasing peptide presentation in mouse dendritic cells.
Jagannath C, Lindsey DR, Dhandayuthapani S, Xu Y, Hunter RL Jr, Eissa NT.
Nat Med. 2009 Mar;15(3):267-76. Epub 2009 Mar 1.
PMID: 19252503
ワクチンの効果を増強

RNA polymerase IV functions in paramutation in Zea mays.
Erhard KF Jr, Stonaker JL, Parkinson SE, Lim JP, Hale CJ, Hollick JB.
Science. 2009 Feb 27;323(5918):1201-5.
PMID: 19251626
小麦のRNApol IVの機能


Epigenetic reprogramming and small RNA silencing of transposable elements in pollen.
Slotkin RK, Vaughn M, Borges F, Tanurdzi? M, Becker JD, Feijo JA, Martienssen RA.
Cell. 2009 Feb 6;136(3):461-72.
PMID: 19203581
トランスポゾンを抑制するsiRNA


miRNA, siRNA, piRNA: Knowns of the unknown.
Kutter C, Svoboda P.
RNA Biol. 2008 Oct 16;5(4).
PMID: 19182524
レビュー

Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms.
Ruiz-Canada C, Kelleher DJ, Gilmore R.
Cell. 2009 Jan 23;136(2):272-83.
PMID: 19167329
isoformごとにノックダウン


Small silencing RNAs: an expanding universe.
Ghildiyal M, Zamore PD.
Nat Rev Genet. 2009 Feb;10(2):94-108.
PMID: 19148191
レビュー

A novel protein, MAPO1, that functions in apoptosis triggered by O(6)-methylguanine mispair in DNA.
Komori K, Takagi Y, Sanada M, Lim TH, Nakatsu Y, Tsuzuki T, Sekiguchi M, Hidaka M.
Oncogene. 2009 Jan 12.
PMID: 19137017
DNA損傷とのミスペアで起こるアポトーシス

Effective treatment of respiratory alphaherpesvirus infection using RNA interference.
Fulton A, Peters ST, Perkins GA, Jarosinski KW, Damiani A, Brosnahan M, Buckles EL, Osterrieder N, Van de Walle GR.
PLoS ONE. 2009;4(1):e4118. Epub 2009 Jan 5.
PMID: 19122813


Regulation of small RNA accumulation in the maize shoot apex.
Nogueira FT, Chitwood DH, Madi S, Ohtsu K, Schnable PS, Scanlon MJ, Timmermans MC.
PLoS Genet. 2009 Jan;5(1):e1000320. Epub 2009 Jan 2.
PMID: 19119413
葉の形成とsiRNAの蓄積

Transfer of small interfering RNA by single-cell electroporation in cerebellar cell cultures.
Tanaka M, Yanagawa Y, Hirashima N.
J Neurosci Methods. 2008 Dec 9.
PMID: 19114056
目的の一つの細胞にのみsiRMAを導入

Identification of a molecular signaling network that regulates a cellular necrotic cell death pathway.
Hitomi J, Christofferson DE, Ng A, Yao J, Degterev A, Xavier RJ, Yuan J.
Cell. 2008 Dec 26;135(7):1311-23.
PMID: 19109899
ネクロプトーシスのシグナル

A safer and more effective strategy for siRNA delivery.
Corbin I, Scott B, Zheng G.
Nanomed. 2009 Jan;4(1):8-9. No abstract available.
PMID: 19108349
レビュー








NEWS