バイオ研究とバイオ研究者の情報チャンネル
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2006年分
siRNA

9216

Nucleic Acids Res. 2006 Dec 14
More complete gene silencing by fewer siRNAs: transparent optimized design and biophysical signature.
設計ルール

Nucleic Acids Symp Ser (Oxf). 2006;(50):243-4.
The factors that contribute to the long-term expression of siRNA.
この号にsiRNA特集あり

BMC Bioinformatics. 2006 Nov 30;7(1):520
An accurate and interpretable model for siRNA efficacy prediction.


J Biol Chem. 2006 Nov 15
L2dtl is essential for cell survival and nuclear division in early mouse embryonic development. ノックアウトでは胎生致死になる遺伝子を、siRNA発現ベクターを受精卵に入れて解析

Genes Dev. 2006 Nov 3
Direct interaction between DNMT1and G9a coordinates DNA and histone methylation during replication.
DNAとヒストンのメチル化が協調している

Genes Dev. 2006 Nov 1;20(21):2985-95 The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster.
アルゴノート2の欠損で、ウィルスに感染しやすくなる。

Nat Biotechnol. 2006 Oct 22
Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance.
miRNAの発現で、ウィルス抵抗性になる

Science. 2006 Oct 13;314(5797):294-7
CDK2-dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage.
CDK2依存の細胞死に関与する新しい遺伝子をノックダウンすると細胞死が減る。非常にきれいな証明です。

Hum Gene Ther. 2006 Sep 25
Intravenous Delivery of Anti-RhoA Small Interfering RNA Loaded in Nanoparticles of Chitosan in Mice: Safety and Efficacy in Xenografted Aggressive Breast Cancer.
キトサンのナノパーティクルによるsiRNA輸送で、がんを治療

Nucleic Acids Res. 2006 Sep 18
Controlling activation of the RNA-dependent protein kinase by siRNAs using site-specific chemical modification.
PKRを誘導しないためのsiRNAセンス鎖の修飾

Curr Biol. 2006 Aug 30
The Role of PIWI and the miRNA Machinery in Drosophila Germline Determination.


Nat Methods. 2006 Sep;3(9):670-6
On the art of identifying effective and specific siRNAs.
siRNA選択のガイドライン

Science. 2006 Aug 25;313(5790):1134-7.
Argonaute slicing is required for heterochromatic silencing and spreading.


Proc Natl Acad Sci U S A. 2006 Aug 17
A2A adenosine receptor protects tumors from antitumor T cells.
siRNAの処理で、がんの免疫療法が効きやすくなる。

RNA. 2006 Jul 26
Efficient prediction of siRNAs with siRNArules 1.0: An open-source JAVA approach to siRNA algorithms.


Nat Neurosci. 2006 Jul 16
IGF-1 receptor is essential for the establishment of hippocampal neuronal polarity.
ニューロンの極性をRNAiで調節

Gene Ther. 2006 Jul 6
Single-walled carbon nanotubes-mediated in vivo and in vitro delivery of siRNA into antigen-presenting cells.
Yang R, Yang X, Zhang Z, Zhang Y, Wang S, Cai Z, Jia Y, Ma Y, Zheng C, Lu Y, Roden R, Chen Y.
カーボンナノチューブによるsiRNAの導入

Genes Dev. 2006 Jul 1;20(13):1715-20.
A positive feedback loop couples Ras activation and CD44 alternative splicing.
Cheng C, Yaffe MB, Sharp PA.
スプライシングバリアントだけをsiRNAで抑える

Science. 2006 Jun 29
A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline.
Vagin VV, Sigova A, Li C, Seitz H, Gvozdev V, Zamore PD.
新しいRNA、rasiRNA

Curr Top Med Chem. 2006;6(9):913-25.
Chemical and structural diversity of siRNA molecules.
Nawrot B, Sipa K.
様々な修飾siRNA。レビュー
Curr Top Med Chem. 2006;6(9):893-900.
RNA Interference with chemically modified siRNA.
Zhang HY, Du Q, Wahlestedt C, Liang Z.
この号に特集あり

Cell. 2006 Jun 2;125(5):873-86.
Tethering RITS to a Nascent Transcript Initiates RNAi- and Heterochromatin-Dependent Gene Silencing.
Buhler M, Verdel A, Moazed D.
RNAi活性に必要なヘテロクロマチン状態

Nucleic Acids Res. 2006 Jun 1;34(10):e73.
Aptamer mediated siRNA delivery.
Chu TC, Twu KY, Ellington AD, Levy M.
細胞特異的アプタマーを使ってsiRNAを導入

Nat Genet. 2006 May 14;
Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning.
Henderson IR, Zhang X, Lu C, Johnson L, Meyers BC, Green PJ, Jacobsen SE.
植物でのDICERの役割

Nucleic Acids Res. 2006 May 11;34(8):2294-304. Print 2006.
Improved targeting of miRNA with antisense oligonucleotides.
Davis S, Lollo B, Freier S, Esau C.
アンチセンスオリゴで、miRNAの働きを阻害する

Biotechniques. 2006 Apr;Suppl:7-13.
Therapeutic potential of siRNA-mediated transcriptional gene silencing.
Morris KV.

Bioorg Med Chem Lett. 2006 Apr 5
RNA interference by 2',5'-linked nucleic acid duplexes in mammalian cells.
Prakash TP, Kraynack B, Baker BF, Swayze EE, Bhat B.


IDrugs. 2006 Apr;9(4):279-82.
Using RNAi in the clinic.
Dev KK.
レビュー

Expert Opin Ther Targets. 2006 Apr;10(2):337-340.
RNAi: for functional analysis and target validation.
Rondinone CM.
最近の事情

Nucleic Acids Res. 2006 Mar 6;34(5):1369-80. Print 2006.
In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5.
Ohrt T, Merkle D, Birkenfeld K, Echeverri CJ, Schwille P.
siRNAとmiRNAを見分ける。exportin5がsiRNAの核内輸送に重要。


Science. 2006 Feb 24;311(5764):1118-23.
Noncoding RNAs of Trithorax Response Elements Recruit Drosophila Ash1 to Ultrabithorax.
Sanchez-Elsner T, Gou D, Kremmer E, Sauer F.
ホメオボックス遺伝子の発現を調節するncRNA

Methods Enzymol. 2006;406:374-88.
Nucleofection of primary neurons.
Gartner A, Collin L, Lalli G.
ニューロンへのエレクトロポレーション

J Biomol Tech. 2005 Sep;16(3):224-34.
Nucleic acid aptamers for target validation and therapeutic applications.
Pendergrast PS, Marsh HN, Grate D, Healy JM, Stanton M.
アプタマーに関するレビューです。


Bioinformatics. 2006 Jan 29
siRecords: an extensive database of mammalian siRNAs with efficacy ratings.
Ren Y, Gong W, Xu Q, Zheng X, Lin D, Wang Y, Li T.
実験で効果の確かめられたsiRNAのデータベースです。
http://siRecords.umn.edu/siRecords/


Virus Res.
2006 Jan 16
EsiRNAs inhibit Hepatitis B virus replication in mice model more efficiently than synthesized siRNAs.
Xuan B, Qian Z, Hong J, Huang W.
EsiRNAのほうが、化学合成したsiRNAよりも効果があるという論文です。しかしこれほど強烈に効くのであれば、もう少しジャーナルのランクが高くても良いような気がします。まずはお試しあれ。

Bioconjug Chem. 2006 Jan 18;17(1):241-244.
Comparative Evaluation of Target-Specific GFP Gene Silencing Efficiencies for Antisense ODN, Synthetic siRNA, and siRNA Plasmid Complexed with PEI-PEG-FOL Conjugate.
Kim SH, Mok H, Jeong JH, Kim SW, Park TG.
様々な方法で、オリゴやプラスミドをトランスフェクションしてみたという論文です。




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