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shRNA
2006年分

511

Nucleic Acids Res. 2006 Dec 7
Development of a species-specific RNA polymerase I-based shRNA expression vector.
polIプロモーターを持つ発現ベクター

Biochem Biophys Res Commun. 2006 Nov 2
Differentiation stage-specific analysis of gene function with inducible short hair-pin RNA in differentiating embryonic stem cells.
ES細胞において、テトラサイクリンでshRNAの発生ステージ特異的な発現を誘導

Oligonucleotides. 2006 Fall;16(3):263-74.
Complete Reversal of ABCG2-Depending Atypical Multidrug Resistance by RNA Interference in Human Carcinoma Cells.
がん細胞の多剤耐性をRNAiで抑える

Methods Mol Biol. 2006;342:287-93
Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector.
レンチウィルスを使った系

Nucleic Acids Res. 2006 Aug 31
Tumor inhibition by genomically integrated inducible RNAi-cassettes.
テトラサイクリンで誘導されるプロモーターで発現

EMBO J. 2006 Aug 17
Identification of CD63 as a tissue inhibitor of metalloproteinase-1 interacting cell surface protein.
two-hybridで取れた遺伝子をshRNAで抑制してみると

Biochem Biophys Res Commun. 2006 Sep 1;347(3):543-50.
A technique to enzymatically construct libraries which express short hairpin RNA of arbitrary stem length.


Blood. 2006 Jul 20
Lentivirus vectors expressing short hairpin RNAs against the U3-overlapping region of HIV nef inhibit HIV replication and infectivity in primary macrophages.
shRNAでHIVの増殖を阻害

Antiviral Res. 2006 Jun 28
Hepatitis B virus is inhibited by RNA interference in cell culture and in mice.
Ying RS, Zhu C, Fan XG, Li N, Tian XF, Liu HB, Zhang BX.


Cancer Gene Ther. 2006 Jun 30
Inhibition of cervical cancer cell growth in vitro and in vivo with lentiviral-vector delivered short hairpin RNA targeting human papillomavirus E6 and E7 oncogenes.
Gu W, Putral L, Hengst K, Minto K, Saunders NA, Leggatt G, McMillan NA.
shRNAで、がん遺伝子を抑制

Nature. 2006 Jun 11
Dissecting self-renewal in stem cells with RNA interference.
Ivanova N, Dobrin R, Lu R, Kotenko I, Levorse J, Decoste C, Schafer X, Lun Y, Lemischka IR.
shRNAで幹細胞をコントロールする

Oligonucleotides. 2006 Summer;16(2):115-144.
Conserved MicroRNA Characteristics in Mammals.
Saetrom P, Snove O, Nedland M, Grunfeld TB, Lin Y, Bass MB, Canon JR.
共通配列を持ったマイクロRNA

Nat Biotechnol. 2006 Jun;24(6):697-702. Epub 2006 May 14.
Short hairpin RNA-expressing bacteria elicit RNA interference in mammals.
Xiang S, Fruehauf J, Li CJ.
shRNAを発現するバクテリアが感染するだけで、宿主にRNAiがおきる

Nature. 2006 May 4;441(7089):106-10. Epub 2006 Mar 29.
Comment in: A loss-of-function RNA interference screen for molecular targets in cancer.
Ngo VN, Davis RE, Lamy L, Yu X, Zhao H, Lenz G, Lam LT, Dave S, Yang L, Powell J, Staudt LM.
shRNAのスクリーニング

Cell. 2006 Mar 24;124(6):1283-98.
A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen.
Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini DM, Root DE.
レンチウィルスによるshRNAライブラリー

Nat Genet. 2005 Nov;37(11):1281-8. Epub 2005 Oct 2.
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