shRNA
20101004
2742
Phosphorylation by the c-Abl protein tyrosine kinase inhibits
parkin's ubiquitination and protective function.
Ko HS, Lee Y, Shin JH, Karuppagounder SS, Gadad BS, Koleske
AJ, Pletnikova O, Troncoso JC, Dawson VL, Dawson TM.
Proc
Natl Acad Sci U S A. 2010 Sep 7. [Epub ahead of print]PMID: 20823226
パーキンソン病遺伝子と関連する遺伝子をノックダウン
Bacterial Delivery of RNAi Effectors: Transkingdom
RNAi.
Lage H, Kr 252 Hn A.
J Vis Exp. 2010 Aug
18;(42). pii: 2099. doi: 10.3791/2099.PMID: 20811323
バクテリアを使って、shRNAを細胞内に輸送
Chitosan/Short hairpin RNA complexes for vascular endothelial
growth factor suppression invasive breast carcinoma.
Salva E, Kabasakal L, Eren F, Cakala?ao?lu F, Ozkan N, Akbu?a
J.
Oligonucleotides. 2010
Aug;20(4):183-90.PMID: 20707740
キトサンとshRNAとの複合体をがんに投与
miR-451
protects against erythroid oxidant stress by repressing 14-3-3{zeta}.
Yu D, Dos Santos CO, Zhao G, Jiang J, Amigo JD, Khandros E,
Dore LC, Yao Y, D'Souza J, Zhang Z, Ghaffari S, Choi J, Friend S, Tong W, Orange
JS, Paw BH, Weiss MJ.
Genes Dev. 2010 Aug
1;24(15):1620-33.PMID: 20679398
赤血球で発現するmiRNA
In
Vivo Application of an RNAi Strategy for the Selective Suppression of a
Mutant Allele.
Kubodera T, Yamada H, Anzai M, Ohira S, Yokota S, Hirai Y,
Mochizuki H, Shimada T, Mitani T, Mizusawa H, Yokota T.
Hum
Gene Ther. 2010 Jul 22. [Epub ahead of print]PMID: 20649474
突然変異した遺伝子の発現を抑制するin vivoでのRNAi
RNAi
screen for telomerase reverse transcriptase transcriptional regulators
identifies HIF1{alpha} as critical for telomerase function in murine embryonic
stem cells.
Coussens M, Davy P, Brown L, Foster C, Andrews WH, Nagata M,
Allsopp R.
Proc
Natl Acad Sci U S A. 2010 Jul 19. [Epub ahead of print]PMID: 20643931
ES細胞において、テロメラーゼの発現に関与する因子
Subtype-specific genomic alterations define new targets for
soft-tissue sarcoma therapy.
Barretina J, Taylor BS, Banerji S, Ramos AH, Lagos-Quintana M,
Decarolis PL, Shah K, Socci ND, Weir BA, Ho A, Chiang DY, Reva B, Mermel CH,
Getz G, Antipin Y, Beroukhim R, Major JE, Hatton C, Nicoletti R, Hanna M, Sharpe
T, Fennell TJ, Cibulskis K, Onofrio RC, Saito T, Shukla N, Lau C, Nelander S,
Silver SJ, Sougnez C, Viale A, Winckler W, Maki RG, Garraway LA, Lash A,
Greulich H, Root DE, Sellers WR, Schwartz GK, Antonescu CR, Lander ES, Varmus
HE, Ladanyi M, Sander C, Meyerson M, Singer S.
Nat
Genet. 2010 Jul 4. [Epub ahead of print]PMID:
20601955
サルコーマで突然変異している遺伝子をshRNAで治療
Oncoproteomic analysis reveals co-upregulation of RELA and
STAT5 in carboplatin resistant ovarian carcinoma.
Jinawath N, Vasoontara C, Jinawath A, Fang X, Zhao K, Yap KL,
Guo T, Lee CS, Wang W, Balgley BM, Davidson B, Wang TL, Shih IeM.
PLoS One.
2010 Jun 18;5(6):e11198.PMID: 20585448
抗がん剤耐性がん細胞の遺伝子発現
PDGF-CC
blockade inhibits pathological angiogenesis by acting on multiple cellular and
molecular targets.
Hou X, Kumar A, Lee C, Wang B, Arjunan P, Dong L, Maminishkis
A, Tang Z, Li Y, Zhang F, Zhang SZ, Wardega P, Chakrabarty S, Liu B, Wu Z,
Colosi P, Fariss RN, Lennartsson J, Nussenblatt R, Gutkind JS, Cao Y, Li X.
Proc
Natl Acad Sci U S A. 2010 Jun 21. [Epub ahead of print]PMID: 20566880
血管新生（阻害）の新しいターゲット
Liposome-siRNA-Peptide Complexes Cross the Blood-Brain
Barrier and Significantly Decrease PrP on Neuronal Cells and PrP in Infected
Cell Cultures.
Pulford B, Reim N, Bell A, Veatch J, Forster G, Bender H,
Meyerett C, Hafeman S, Michel B, Johnson T, Wyckoff AC, Miele G, Julius C,
Kranich J, Schenkel A, Dow S, Zabel MD.
PLoS One.
2010 Jun 14;5(6):e11085.PMID: 20559428
リポソームとsiRNAとペプチドの細胞への導入
Albumin
expression is required for adipocyte differentiation of 3T3-L1 cells.
Yoo W, Lee J, Park S, Kim YS, Lim C, Oh J.
Biochem Biophys Res
Commun. 2010 May 28. [Epub ahead of print]PMID:
20529675
アルブミンの発現と脂肪の蓄積の相関
Intra-articular lentivirus-mediated delivery of galectin-3
shRNA and galectin-1 gene ameliorates collagen-induced arthritis.
Wang CR, Shiau AL, Chen SY, Cheng ZS, Li YT, Lee CH, Yo YT, Lo
CW, Lin YS, Juan HY, Chen YL, Wu CL.
Gene
Ther. 2010 Jun 3. [Epub ahead of print]PMID:
20520649
shRNAで関節炎を治療
Nuclear factor-{kappa}B signaling and ezrin are essential
for L1-mediated metastasis of colon cancer cells.
Gavert N, Ben-Shmuel A, Lemmon V, Brabletz T, Ben-Ze'ev A.
J Cell Sci. 2010 May 25. [Epub ahead of
print]PMID: 20501702
NF-kBの発現と、大腸がんの転移
SiRNA-loaded multi-shell nanoparticles incorporated into a
multilayered film as a reservoir for gene silencing.
Zhang X, Kovtun A, Mendoza-Palomares C, Oulad-Abdelghani M,
Fioretti F, Rinckenbach S, Mainard D, Epple M, Benkirane-Jessel N.
Biomaterials. 2010 May 18. [Epub ahead of
print]PMID: 20488536
siRNAをフィルムで投与
Optimization of duplex stability and terminal asymmetry for
shRNA design.
Matveeva OV, Kang Y, Spiridonov AN, Saetrom P, Nemtsov VA,
Ogurtsov AY, Nechipurenko YD, Shabalina SA.
PLoS One. 2010 Apr 20;5(4):e10180.PMID: 20422034
shRNA設計サイト
Function-based gene identification using enzymatically generated normalized
shRNA library and massive parallel sequencing.
Shtutman M, Maliyekkel A, Shao Y, Carmack CS, Baig M, Warholic
N, Cole K, Broude EV, Harkins TT, Ding Y, Roninson IB.
Proc
Natl Acad Sci U S A. 2010 Apr 5. [Epub ahead of print]PMID: 20368428
shRNAのライブラリー
Reduced seed region-based off-target activity with
lentivirus-mediated RNAi.
Klinghoffer RA, Magnus J, Schelter J, Mehaffey M, Coleman C,
Cleary MA.
RNA. 2010 Mar 26. [Epub ahead of
print]PMID: 20348445
オリゴよりもshRNAの方が、特異性が高い
A miR-21 hairpin structure-based gene knockdown
vector.
Yue J, Sheng Y, Ren A, Penmatsa S.
Biochem Biophys Res
Commun. 2010 Mar 11. [Epub ahead of print]PMID:
20226761
ベクターの開発
Optimization of shRNA inhibitors by variation of the
terminal loop sequence.
Schopman NC, Liu YP, Konstantinova P, Ter Brake O, Berkhout
B.
Antiviral Res. 2010 Feb 24. [Epub ahead of
print]PMID: 20188764
shRNAのループ配列の検討
Novel HIV-1 Knockdown Targets Identified by an Enriched
Kinases/Phosphatases shRNA Library Using a Long-Term Iterative Screen in Jurkat
T-Cells.
Rato S, Maia S, Brito PM, Resende L, Pereira CF, Moita C,
Freitas RP, Moniz-Pereira J, Hacohen N, Moita LF, Goncalves J.
PLoS One. 2010 Feb 17;5(2):e9276.PMID: 20174665
shRNAライブラリーを使用
Cell type specificity of chromatin organization mediated by CTCF and
cohesin.
Hou C, Dale R, Dean A.
Proc
Natl Acad Sci U S A. 2010 Feb 2. [Epub ahead of print]PMID: 20133600
CTCFとcohesinによるクロマチン構造
Efficient Downregulation of Multiple mRNA Targets with a Single
shRNA-Expressing Lentiviral Vector.
Chumakov SP, Kravchenko JE, Prassolov VS, Frolova EI, Chumakov
PM.
Plasmid. 2010 Jan 8. [Epub ahead of print]PMID: 20064551
複数のshRNAを発現
shRNA targeting HDGF suppressed cell growth and invasion of squamous
cell lung cancer.
Meng J, Xie W, Cao L, Hu C, Zhe Z.
Acta Biochim Biophys Sin
(Shanghai). 2010 Jan;42(1):52-7.PMID: 20043047
がんを治療するshRNA
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