oligonucleotide 20091222
202734
Human
POT1 is required for efficient telomere C-rich strand replication in the absence
of WRN.
Arnoult N, Saintome C, Ourliac-Garnier I, Riou JF,
Londono-Vallejo A.
Genes Dev. 2009 Dec
15;23(24):2915-24.PMID: 20008939
In
vivo selection of tumor-targeting RNA motifs.
Mi J, Liu Y, Rabbani ZN, Yang Z, Urban JH, Sullenger BA, Clary
BM.
Nat Chem Biol. 2010 Jan;6(1):22-4. Epub
2009 Nov 29.PMID: 19946274
RNAアプタマー
Therapeutic
Silencing of MicroRNA-122 in Primates with Chronic Hepatitis C Virus
Infection.
Lanford RE, Hildebrandt-Eriksen ES, Petri A, Persson R, Lindow
M, Munk ME, Kauppinen S, Orum H.
Science. 2009 Dec 3. [Epub ahead of
print]PMID: 19965718
LNAでmiRNAを抑える
Sequence-Specific
Correction of Genomic HPRT Mutations in Lymphoblasts by Small Fragment
Homologous Replacement (SFHR).
Bedayat B, Abdolmohamadi A, Ye L, Maurisse R, Parsi H, Schwarz
J, Emamekhoo H, Nicklas JA, Oneill JP, Gruenert DC.
Oligonucleotides. 2009 Dec 8. [Epub ahead of
print]PMID: 19995283
突然変異を修復する実験系を開発
Tuning
the cellular uptake and cytotoxicity properties of oligonucleotide
intercalator-functionalized mesoporous silica nanoparticles with human cervical
cancer cells HeLa.
Vivero-Escoto JL, Slowing II, Lin VS.
Biomaterials. 2009 Nov 23.
PMID: 19932923
オリゴ導入のためのナノパーティクル
Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides.
Bertoni C, Rustagi A, Rando TA.
Nucleic Acids Res. 2009 Oct 23.
PMID: 19854937
5メチルシトシンを含む（3'端はｃｇｃｇのS化、5端はCY3で標識'）1本鎖DNAを鋳型として、細胞中の遺伝子の一塩基を、突然変異できる、遺伝子を高効率に修復できる、という論文。MBD4遺伝子は、ミスマッチ修復に関与する可能性も。修復機構とオリゴを使って、ゲノム中の遺伝子の一塩基置換を修復できる可能性が高まりました。これはすごい論文かもしれません。
In ovo application of antagomiRs indicates a role for
miR-196 in patterning the chick axial skeleton through Hox gene
regulation.
McGlinn E, Yekta S, Mansfield JH, Soutschek J, Bartel DP, Tabin
CJ.
Proc
Natl Acad Sci U S A. 2009 Oct 21
PMID: 19846767
antagomiRsというアンチセンスオリゴを利用
The HSV-1 ICP27 RGG box specifically binds flexible,
GC-rich sequences but not G-quartet structures.
Corbin-Lickfett KA, Chen IH, Cocco MJ, Sandri-Goldin RM.
Nucleic
Acids Res. 2009 Sep 26.
PMID: 19783816
GCリッチ配列に結合する、RNA結合ペプチド
Origins and functional impact of copy number variation
in the human genome.
Conrad DF, Pinto D, Redon R, Feuk L, Gokcumen O, Zhang Y, Aerts
J, Andrews TD, Barnes C, Campbell P, Fitzgerald T, Hu M, Ihm CH, Kristiansson K,
Macarthur DG, Macdonald JR, Onyiah I, Pang AW, Robson S, Stirrups K, Valsesia A,
Walter K, Wei J; The Wellcome Trust Case Control Consortium, Tyler-Smith C,
Carter NP, Lee C, Scherer SW, Hurles ME.
Nature. 2009 Oct 7.
PMID: 19812545
ゲノム内の遺伝子の増幅を網羅
Recent Patents in Antiviral siRNAs.
Saravolac EG, Wong JP, Cairns MJ.
Recent Pat Antiinfect
Drug Discov. 2009 Oct 7.
PMID: 19807677
siRNA関連の特許
Direct RNA sequencing.
Ozsolak F, Platt AR, Jones DR, Reifenberger JG, Sass LE,
McInerney P, Thompson JF, Bowers J, Jarosz M, Milos PM.
Nature. 2009 Sep 23.
PMID: 19776739
RNAの配列を直接解析する
Oncogene homologue Sch9 promotes age-dependent
mutations by a superoxide and Rev1/Polzeta-dependent mechanism.
Madia F, Wei M, Yuan V, Hu J, Gattazzo C, Pham P, Goodman MF,
Longo VD.
J
Cell Biol. 2009 Aug 24;186(4):509-23. Epub 2009 Aug 17.
PMID: 19687253
加齢とともに突然変異を増やす、がん遺伝子に似た遺伝子
Targeted gene conversion induced by triplex-directed
psoralen interstrand crosslinks in mammalian cells.
Liu Y, Nairn RS, Vasquez KM.
Nucleic
Acids Res. 2009 Sep 2.
PMID: 19726585
ゲノム中の特定の遺伝子を組換える方法
CpG methylation potentiates pixantrone and
doxorubicin-induced DNA damage and is a marker of drug sensitivity.
Evison BJ, Bilardi RA, Chiu FC, Pezzoni G, Phillips DR, Cutts
SM.
Nucleic
Acids Res. 2009 Aug 31.
PMID: 19720735
CpGメチル化と薬剤感受性
Zip Nucleic Acids: new high affinity oligonucleotides
as potent primers for PCR and reverse transcription.
Moreau V, Voirin E, Paris C, Kotera M, Nothisen M, Remy JS,
Behr JP, Erbacher P, Lenne-Samuel N.
Nucleic
Acids Res. 2009 Aug 20.
PMID: 19696078
Triplet-repeat
oligonucleotide-mediated reversal of RNA toxicity in myotonic dystrophy.
Mulders SA, van den Broek WJ, Wheeler TM, Croes HJ, van
Kuik-Romeijn P, de Kimpe SJ, Furling D, Platenburg GJ, Gourdon G, Thornton CA,
Wieringa B, Wansink DG.
Proc
Natl Acad Sci U S A. 2009 Aug 18;106(33):13915-20. Epub 2009 Aug 10.
PMID: 19667189
2'-O-メチルのCAGリピートアンチセンスオリゴが、CAGリピート病原因遺伝子を阻害する
Targeted
inhibition of Snail family zinc finger transcription factors by
oligonucleotide-Co(III) Schiff base conjugate.
Harney AS, Lee J, Manus LM, Wang P, Ballweg DM, Labonne C,
Meade TJ.
Proc
Natl Acad Sci U S A. 2009 Aug 3. [Epub ahead of print]
PMID: 19666616
オリゴで転写因子を阻害
An
alternative DNA structure is necessary for pilin antigenic variation in
Neisseria gonorrhoeae.
Cahoon LA, Seifert HS.
Science. 2009 Aug 7;325(5941):764-7.
PMID: 19661435
組み換えを引き起こすグアニンリッチ配列
An
alternative DNA structure is necessary for pilin antigenic variation in
Neisseria gonorrhoeae.
Cahoon LA, Seifert HS.
Science. 2009 Aug 7;325(5941):764-7.
PMID: 19661435
有害な繰り返し配列をもつmRNAをアンチセンスオリゴで無毒化
An
integrated genome screen identifies the Wnt signaling pathway as a major target
of WT1.
Kim MK, McGarry TJ, O Broin P, Flatow JM, Golden AA, Licht
JD.
Proc
Natl Acad Sci U S A. 2009 Jul 7;106(27):11154-9. Epub 2009 Jun 22.
PMID: 19549856
ChIP-ChIPで、転写産物をスクリーニング
SirT1
knockdown in liver decreases basal hepatic glucose production and increases
hepatic insulin responsiveness in diabetic rats.
Erion DM, Yonemitsu S, Nie Y, Nagai Y, Gillum MP, Hsiao JJ,
Iwasaki T, Stark R, Weismann D, Yu XX, Murray SF, Bhanot S, Monia BP, Horvath
TL, Gao Q, Samuel VT, Shulman GI.
Proc
Natl Acad Sci U S A. 2009 Jul 7;106(27):11288-93. Epub 2009 Jun 22.
PMID: 19549853
グルコースの代謝を調節するRNAi
Detection
of cancer with serum miRNAs on an oligonucleotide microarray.
Lodes MJ, Caraballo M, Suciu D, Munro S, Kumar A, Anderson
B.
PLoS One. 2009
Jul 14;4(7):e6229.
PMID: 19597549
がん細胞特有のmiRNA発現パターンをマイクロアレイで検出
NPAS3
is a trachealess homolog critical for lung development and homeostasis.
Zhou S, Degan S, Potts EN, Foster WM, Sunday ME.
Proc
Natl Acad Sci U S A. 2009 Jul 6.
PMID: 19581591
肺の発生に関与する転写因子をクローニング
Protein-free
small nuclear RNAs catalyze a two-step splicing reaction.
Valadkhan S, Mohammadi A, Jaladat Y, Geisler S.
Proc
Natl Acad Sci U S A. 2009 Jun 22.
PMID: 19549866
U6とU2のRNA複合体に、リボザイム活性がある
MLL
associates with telomeres and regulates telomeric repeat-containing RNA
transcription.
Caslini C, Connelly JA, Serna A, Broccoli D, Hess JL.
Mol Cell Biol. 2009 Jun 15.
PMID: 19528237
MLLタンパク質が、テロメア領域のヒストンのメチル化や転写を調節する
(micro)genomic
medicine: microRNAs as therapeutics and biomarkers.
Saunders MA, Lim LP.
RNA Biol. 2009 Jul 29;6(3).
PMID: 19502816
レビュー
Preparation
of short interfering RNA containing the modified nucleosides 2-thiouridine,
pseudouridine, or dihydrouridine.
Nawrot B, Sochacka E.
Curr
Protoc Nucleic Acid Chem. 2009 Jun;Chapter 16:Unit 16.2.
PMID: 19488969
特殊塩基のsiRNA
Identification of the pollen self-incompatibility determinant in Papaver
rhoeas.
Wheeler MJ, de Graaf BH, Hadjiosif N, Perry RM, Poulter NS,
Osman K, Vatovec S, Harper A, Franklin FC, Franklin-Tong VE.
Nature. 2009 May 31.
PMID: 19483678
アンチセンスで、自家不和合性を解消
Increasing
the relative expression of endogenous non-coding Steroid Receptor RNA Activator
(SRA) in human breast cancer cells using modified oligonucleotides.
Cooper C, Guo J, Yan Y, Chooniedass-Kothari S, Hube F, Hamedani
MK, Murphy LC, Myal Y, Leygue E.
Nucleic
Acids Res. 2009 May 29.
PMID: 19483093
アンチセンスオリゴで、スプライシングを調節する
Mismatch
repair and nucleotide excision repair proteins cooperate in the recognition of
DNA interstrand crosslinks.
Zhao J, Jain A, Iyer RR, Modrich PL, Vasquez KM.
Nucleic
Acids Res. 2009 May 25.
PMID: 19468048
ソラーレンの結合した3重鎖オリゴを、修復するシステム
A
yeast hybrid provides insight into the evolution of gene expression
regulation.
Tirosh I, Reikhav S, Levy AA, Barkai N.
Science. 2009 May
1;324(5927):659-62.
PMID: 19407207
遺伝子発現調節機構の進化
Oligonucleotides
suppress PKB/Akt and act as superinductors of apoptosis in human
keratinocytes.
Kippenberger S, Muller J, Schultz M, Dorn A, Bock A, Aygun H,
Thaci D, Hofmann M, Kaufmann R, Bernd A.
Nucleic
Acids Res. 2009 Apr 22.
PMID: 19386618
アポトーシスを引き起こすオリゴ
Imaging
intracellular RNA distribution and dynamics in living cells.
Tyagi S.
Nat
Methods. 2009 May;6(5):331-8.
PMID: 19404252
生きた細胞で、mRNAの挙動を観察する
Self-association
of short DNA loops through minor groove C:G:G:C tetrads.
Viladoms J, Escaja N, Frieden M, Gomez-Pinto I, Pedroso E,
Gonzalez C.
Nucleic
Acids Res. 2009 Mar 24.
PMID: 19321501
CGとGCのところでループを形成する
Getting
a handle on Huntington's disease: silencing neurodegeneration.
La Spada AR.
Nat Med.
2009 Mar;15(3):252-3. No abstract available.
PMID: 19265826
コメント
A
transposon-based genetic screen in mice identifies genes altered in colorectal
cancer.
Starr TK, Allaei R, Silverstein KA, Staggs RA, Sarver AL,
Bergemann TL, Gupta M, O'Sullivan MG, Matise I, Dupuy AJ, Collier LS, Powers S,
Oberg AL, Asmann YW, Thibodeau SN, Tessarollo L, Copeland NG, Jenkins NA,
Cormier RT, Largaespada DA.
Science. 2009 Mar 27;323(5922):1747-50.
Epub 2009 Feb 26.
PMID: 19251594
さまざまな大腸がんの中の、トランスポゾンによる遺伝子の変化をチェック
Stem-Loop
RT-PCR Quantification of siRNAs In Vitro and In Vivo.
Cheng A, Li M, Liang Y, Wang Y, Wong L, Chen C, Vlassov AV,
Magdaleno S.
Oligonucleotides. 2009 Mar 14.
PMID: 19284311
細胞内のsiRNAの量を測定
Transcriptomics:
The digital generation.
Blow N.
Nature. 2009 Mar 12;458(7235):239-42.
PMID: 19279641
レビュー
Universal
protein-binding microarrays for the comprehensive characterization of the
DNA-binding specificities of transcription factors.
Berger MF, Bulyk ML.
Nat
Protoc. 2009;4(3):393-411.
PMID: 19265799
転写因子を同定するマイクロアレイ
Autocatalytic
aptazymes enable ligand-dependent exponential amplification of RNA.
Lam BJ, Joyce GF.
Nat
Biotechnol. 2009 Feb 22.
PMID: 19234448
RNA enzyme同士の相互作用
Temperature-dependent
FRET spectroscopy for the high-throughput analysis of self-assembled DNA
nanostructures in real time.
Sacca B, Meyer R, Niemeyer CM.
Nat
Protoc. 2009;4(3):271-85.
PMID: 19214179
DNA分子の集合をFRETで検出
A
synthetic snRNA m3G-CAP enhances nuclear delivery of exogenous proteins and
nucleic acids.
Moreno PM, Wenska M, Lundin KE, Wrange O, Stromberg R, Smith
CI.
Nucleic
Acids Res. 2009 Feb 10.
PMID: 19208638
m3G-CAPをつけた核酸やタンパク質の核内への輸送
Regulation
of targeted gene repair by intrinsic cellular processes.
Engstrom JU, Suzuki T, Kmiec EB.
Bioessays. 2009 Feb 9;31(2):159-168.
PMID: 19204988
遺伝病の治療
Repeatability
of published microarray gene expression analyses.
Ioannidis JP, Allison DB, Ball CA, Coulibaly I, Cui X, Culhane
AC, Falchi M, Furlanello C, Game L, Jurman G, Mangion J, Mehta T, Nitzberg M,
Page GP, Petretto E, van Noort V.
Nat
Genet. 2009 Feb;41(2):149-55. Epub 2008 Jan 28.
PMID: 19174838
マイクロアレイデータの比較
Effects
of linker sequences on vesicle fusion mediated by lipid-anchored DNA
oligonucleotides.
Chan YH, van Lengerich B, Boxer SG.
Proc
Natl Acad Sci U S A. 2009 Jan 27;106(4):979-84. Epub 2009 Jan 21.
PMID: 19164559
リンカーとしてもオリゴの配列
RNA
repair restores hemoglobin expression in IVS2-654 thalassemic mice.
Svasti S, Suwanmanee T, Fucharoen S, Moulton HM, Nelson MH,
Maeda N, Smithies O, Kole R.
Proc
Natl Acad Sci U S A. 2009 Jan 27;106(4):1205-10. Epub 2009 Jan 21.
PMID: 19164558
スプライシングを調節するモルフォリノオリゴ
AIM2
activates the inflammasome and cell death in response to cytoplasmic
DNA.
Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES.
Nature. 2009 Jan 21.
PMID: 19158676
細胞死を引き起こす細胞質のDNA
DNA
triplex formation with 5-dimethylaminopropargyl deoxyuridine.
Rusling DA, Peng G, Srinivasan N, Fox KR, Brown T.
Nucleic
Acids Res. 2009 Jan 12.
PMID: 19139069
The
effect of the TRF2 N-terminal and TRFH regions on telomeric G-quadruplex
structures.
Pedroso IM, Hayward W, Fletcher TM.
Nucleic
Acids Res. 2009 Jan 12.
PMID: 19139067
Self-Sustained
Replication of an RNA Enzyme.
Lincoln TA, Joyce GF.
Science. 2009 Jan 8.
PMID: 19131595
Phototoxic
aptamers selectively enter and kill epithelial cancer cells.
Ferreira CS, Cheung MC, Missailidis S, Bisland S, Gariepy
J.
Nucleic
Acids Res. 2008 Dec 22.
PMID: 19103663
Efficient
Gene Knockdowns in Human Embryonic Stem Cells Using Lentiviral-Based
RNAi.
Tulpule A, Daley GQ.
Methods Mol Biol.
2009;482:35-42.
PMID: 19089348
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