バイオ研究とバイオ研究者の情報チャンネル
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oligonucleotide 20100915


211684

Walking of antitumor bifunctional trinuclear PtII complex on double-helical DNA.
Malina J, Kasparkova J, Farrell NP, Brabec V.
Nucleic Acids Res. 2010 Sep 9. [Epub ahead of print]PMID: 20833634
3つのプラチナを持つ抗がん剤


De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis.
Fordyce PM, Gerber D, Tran D, Zheng J, Li H, Derisi JL, Quake SR.
Nat Biotechnol. 2010 Aug 29. [Epub ahead of print]PMID: 20802496
転写因子結合部位を見つける

In vitro assembly of cubic RNA-based scaffolds designed in silico.
Afonin KA, Bindewald E, Yaghoubian AJ, Voss N, Jacovetty E, Shapiro BA, Jaeger L.
Nat Nanotechnol. 2010 Aug 29. [Epub ahead of print]PMID: 20802494
オリゴで作った立方体


Anti-tumor activity of splice-switching oligonucleotides.
Bauman JA, Li SD, Yang A, Huang L, Kole R.
Nucleic Acids Res. 2010 Aug 18. [Epub ahead of print]PMID: 20719743
スプライシングを変化させて、アポトーシスを引き起こす


Sequence-specific cleavage of RNA by Type II restriction enzymes.
Murray IA, Stickel SK, Roberts RJ.
Nucleic Acids Res. 2010 Aug 11. [Epub ahead of print]PMID: 20702422
RNA-DNAハイブリッドにも作用する制限酵素


Antisense oligonucleotides and spinal muscular atrophy: skipping along.
Burghes AH, McGovern VL.
Genes Dev. 2010 Aug 1;24(15):1574-9.PMID: 20679391
アンチセンスオリゴで、スプライシングの異常を回復、遺伝病を治療


Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides.
Warner JR, Reeder PJ, Karimpour-Fard A, Woodruff LB, Gill RT.
Nat Biotechnol. 2010 Jul 18. [Epub ahead of print]PMID: 20639866
バーコードのオリゴを用いて、転写状況を解析


Antisense correction of SMN2 splicing in the CNS rescues necrosis in a type III SMA mouse model.
Hua Y, Sahashi K, Hung G, Rigo F, Passini MA, Bennett CF, Krainer AR.
Genes Dev. 2010 Jul 12. [Epub ahead of print]PMID: 20624852
2'-o-メチルSオリゴを用いて、mRNAのスプライシングを正常化


Double-stranded DNA-templated cleavage of oligonucleotides containing a P3'->N5' linkage triggered by triplex formation: the effects of chemical modifications and remarkable enhancement in reactivity.
Ito KR, Kodama T, Tomizu M, Negoro Y, Orita A, Osaki T, Hosoki N, Tanaka T, Imanishi T, Obika S.
Nucleic Acids Res. 2010 Jul 8. [Epub ahead of print]PMID: 20615902
DNA2本鎖が3重鎖になることで、鎖切断が起きやすくなる

Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates.
Straarup EM, Fisker N, Hedtjarn M, Lindholm MW, Rosenbohm C, Aarup V, Hansen HF, Orum H, Hansen JB, Koch T.
Nucleic Acids Res. 2010 Jul 8. [Epub ahead of print]PMID: 20615897
LNAのアンチセンスで、遺伝子治療ができるかもしれない


Subtle gene modification in mouse ES cells: evidence for incorporation of unmodified oligonucleotides without induction of DNA damage.
Aarts M, Te Riele H.
Nucleic Acids Res. 2010 Jul 2. [Epub ahead of print]PMID: 20601408
ES細胞での突然変異の誘導


Native chemical ligation in the synthesis of internally modified oligonucleotide-peptide conjugates.
Diezmann F, Eberhard H, Seitz O.
Biopolymers. 2010 Jun 30;94(4):397-404. [Epub ahead of print]PMID: 20593471
オリゴの鎖内にペプチドを結合

Aptamers as therapeutics.
Keefe AD, Pai S, Ellington A.
Nat Rev Drug Discov. 2010 Jul;9(7):537-50.PMID: 20592747
アプタマー、レビュー


Therapy development in spinal muscular atrophy.
Sendtner M.
Nat Neurosci. 2010 Jul;13(7):795-9.PMID: 20581815
転写翻訳の異常をオリゴで修正


Intracellular delivery of an antisense oligonucleotide via endocytosis of a G protein-coupled receptor.
Ming X, Alam MR, Fisher M, Yan Y, Chen X, Juliano RL.
Nucleic Acids Res. 2010 Jun 15. [Epub ahead of print]PMID: 20551131
BBNペプチドを融合して、アンチセンスオリゴを細胞に導入

Analysis of CUGBP1 targets identifies GU-repeat sequences that mediate rapid mRNA decay.
Rattenbacher B, Beisang D, Wiesner DL, Jeschke JC, von Hohenberg M, St Louis-Vlasova IA, Bohjanen PR.
Mol Cell Biol. 2010 Jun 14. [Epub ahead of print]PMID: 20547756
GUリピートに結合して、mRNAを分解させる因子


Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology.
Orlando SJ, Santiago Y, Dekelver RC, Freyvert Y, Boydston EA, Moehle EA, Choi VM, Gopalan SM, Lou JF, Li J, Miller JC, Holmes MC, Gregory PD, Urnov FD, Cost GJ.
Nucleic Acids Res. 2010 Jun 8. [Epub ahead of print]PMID: 20530528
ヌクレアーゼと修復を利用して、遺伝子組み換えを行う


Removal of DNA from RNA.
Rio DC, Ares M Jr, Hannon GJ, Nilsen TW.
Cold Spring Harb Protoc. 2010 Jun 1;2010(6):pdb.prot5443.PMID: 20516181
コンタミしたDNAを取り除く方法


Prp43p contains a processive helicase structural architecture with a specific regulatory domain.
Walbott H, Mouffok S, Capeyrou R, Lebaron S, Humbert O, van Tilbeurgh H, Henry Y, Leulliot N.
EMBO J. 2010 May 28. [Epub ahead of print]PMID: 20512115
DNA/RNAヘリケースのドメイン解析

Inhibition of lung tumor growth by complex pulmonary delivery of drugs with oligonucleotides as suppressors of cellular resistance.
Garbuzenko OB, Saad M, Pozharov VP, Reuhl KR, Mainelis G, Minko T.
Proc Natl Acad Sci U S A. 2010 May 24. [Epub ahead of print]PMID: 20498076
抗がん剤とアンチセンスの同時投与


Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations.
Petrov A, Puglisi JD.
Nucleic Acids Res. 2010 May 25. [Epub ahead of print]PMID: 20501598
酵母のリボゾームを標識


miR-10b targets Tiam1: Implications for Rac activation and carcinoma migration.
Moriarty CH, Pursell B, Mercurio AM.
J Biol Chem. 2010 May 5. [Epub ahead of print]PMID: 20444703
がん細胞の集合に関与する、miRNA


Induction of tumour immunity by targeted inhibition of nonsense-mediated mRNA decay.
Pastor F, Kolonias D, Giangrande PH, Gilboa E.
Nature. 2010 May 13;465(7295):227-30.PMID: 20463739
オリゴでがんへの免疫を誘導する


Genome-wide association identifies OBFC1 as a locus involved in human leukocyte telomere biology.
Levy D, Neuhausen SL, Hunt SC, Kimura M, Hwang SJ, Chen W, Bis JC, Fitzpatrick AL, Smith E, Johnson AD, Gardner JP, Srinivasan SR, Schork N, Rotter JI, Herbig U, Psaty BM, Sastrasinh M, Murray SS, Vasan RS, Province MA, Glazer NL, Lu X, Cao X, Kronmal R, Mangino M, Soranzo N, Spector TD, Berenson GS, Aviv A.
Proc Natl Acad Sci U S A. 2010 Apr 26. [Epub ahead of print]PMID: 20421499
テロメアと相互作用する因子


Ab initio gene identification in metagenomic sequences
Zhu W, Lomsadze A, Borodovsky M.
Nucleic Acids Res. 2010 Apr 19. [Epub ahead of print]PMID: 20403810
ゲノム配列から、遺伝子を見つけるアルゴリズム


Separating speed and ability to displace roadblocks during DNA translocation by FtsK.
Crozat E, Meglio A, Allemand JF, Chivers CE, Howarth M, Venien-Bryan C, Grainge I, Sherratt DJ.
EMBO J. 2010 Apr 8. [Epub ahead of print]PMID: 20379135
DNAを移動する仕組み


Robust and specific inhibition of microRNAs in Caenorhabditis elegans.
Kundu ST, Slack FJ.
J Biol. 2010 Apr 1;9(3):20. [Epub ahead of print]PMID: 20359318
miRNAを阻害する

Aptamer-targeted cell-specific RNA interference.
Zhou J, Rossi JJ.
Silenc. 2010 Feb 1;1(1):4.PMID: 20226078
siRNAを細胞特異的に輸送、レビュー


Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process.
Leproust EM, Peck BJ, Spirin K, McCuen HB, Moore B, Namsaraev E, Caruthers MH.
Nucleic Acids Res. 2010 Mar 22. [Epub ahead of print]PMID: 20308161
長いオリゴの脱プリン化を防ぐ合成方法

Immunostimulatory CpG oligonucleotides: Effect on gene expression and utility as vaccine adjuvants.
Klinman DM, Klaschik S, Tomaru K, Shirota H, Tross D, Ikeuchi H.
Vaccine. 2010 Feb 23;28(8):1919-23.PMID: 20188247
CpGオリゴによる、免疫刺激


Efficient inhibition of miR-155 function in vivo by peptide nucleic acids.
Fabani MM, Abreu-Goodger C, Williams D, Lyons PA, Torres AG, Smith KG, Enright AJ, Gait MJ, Vigorito E.
Nucleic Acids Res. 2010 Mar 11. [Epub ahead of print]PMID: 20223773
miRNAをPNAで阻害


Human tRNA-derived small RNAs in the global regulation of RNA silencing.
Haussecker D, Huang Y, Lau A, Parameswaran P, Fire AZ, Kay MA.
RNA. 2010 Feb 24. [Epub ahead of print]PMID: 20181738
tRNAから派生したスモールRNA

Muscling in: Gene therapies for muscular dystrophy target RNA.
Chamberlain JR, Chamberlain JS.
Nat Med. 2010 Feb;16(2):170-1.PMID: 20134472
アンチセンスで筋委縮症を治療

Synthetic design of strong promoters.
Schlabach MR, Hu JK, Li M, Elledge SJ.
Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2538-43. Epub 2010 Jan 21.PMID: 20133776
転写が強力になるエンハンサーを発見


Slow non-specific accumulation of 2'-deoxy and 2'-O-methyl oligonucleotide probes at mitochondria in live cells.
Rhee WJ, Bao G.
Nucleic Acids Res. 2010 Feb 10. [Epub ahead of print]PMID: 20147460
修飾オリゴが次第にミトコンドリアに蓄積される


A sensitive non-radioactive northern blot method to detect small RNAs.
Kim SW, Li Z, Moore PS, Monaghan AP, Chang Y, Nichols M, John B.
Nucleic Acids Res. 2010 Jan 15. [Epub ahead of print]PMID: 20081203
スモールRNAのノザン解析


ATM is down-regulated by N-Myc-regulated microRNA-421.
Hu H, Du L, Nagabayashi G, Seeger RC, Gatti RA.
Proc Natl Acad Sci U S A. 2010 Jan 4. [Epub ahead of print]PMID: 20080624
ATMの発現に関与するmiRNA


Regulatory elements: A metamorphosis in synthesis.
Gunter C.
Nat Rev Genet. 2010 Jan;11(1):4. No abstract available. PMID: 20050273
レビュー

Systems biology: Small genome, complex regulation.
Flintoft L.
Nat Rev Genet. 2010 Jan;11(1):3. No abstract available. PMID: 20050270
レビュー

Enforcing temporal control of maternal mRNA translation during oocyte cell-cycle progression.
Arumugam K, Wang Y, Hardy LL, Macnicol MC, Macnicol AM.
EMBO J. 2009 Dec 3. [Epub ahead of print]PMID: 19959990
母由来mRNAの翻訳















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